DNA phylogeny of the extinct marsupial wolf

  title={DNA phylogeny of the extinct marsupial wolf},
  author={Richard H. Thomas and Walter Schaffner and Allan Charles Wilson and Svante P{\"a}{\"a}bo},
THE phylogenetic affiliation of the extinct marsupial wolf (Thy-lacinus cynocephalus), which once was widespread in Australia, has been uncertain. On the basis of morphology, some systematists argue that the thylacine was most closely related to an extinct group of South American carnivorous marsupials, the borhyaenids1-3, whereas others consider it to be closer to Australian carnivorous marsupials4. Here we use direct sequencing by means of the polymerase chain reaction (PCR) to compare 219… 

DNA phylogeny of the marsupial wolf resolved

Phylogenetic analysis of mitochondrial DNA sequence and nuclear protamine gene sequence shows unambiguously that thylacines are members of Dasyuromorphia, and suggests a late Oligocene or very early Miocene divergence of familial lineages.

Phylogenetic relationships of dasyuromorphian marsupials revisited

The phylogenetic relationships of dasyuromorphians are reassessed using a large molecular database comprising previously published and new sequences for both nuclear (nDNA) and mitochondrial (mtDNA) genes from the numbat, most living species of Dasyuridae, and the recently extinct marsupial wolf, to suggest that Thylacinidae is sister to Myrmecobiidae + Dasyurids.

Phylogenetic relationships of the thylacine (Mammalia: Thylacinidae) among dasyuroid marsupials : evidence from cytochrome b DNA sequences

Phylogenetic analysis of the sequences reported here suggests that the thylacine is a sister group to Dasyuridae and lends support to the hypothesis that Thylacinus represents an ancient Australian marsupial lineage.

Phylogenetic relationships and biogeography of xantusiid lizards, inferred from mitochondrial DNA sequences.

Levels of sequence divergence, as well as the age and affinities of some mainland fossil taxa, suggest that the origin of Cricosaura was associated with the tectonic evolution of the Greater Antilles in the late Cretaceous.

The mitochondrial genome sequence of the Tasmanian tiger (Thylacinus cynocephalus).

The first two complete mitochondrial genome sequences of the thylacine (Thylacinus cynocephalus), or so-called Tasmanian tiger, extinct since 1936 are reported, adding to the growing evidence that extensive sequencing of museum collections is both feasible and desirable, and can yield complete genomes.

Mitochondrial DNA from Myotragus balearicus, an extinct bovid from the Balearic Islands.

Phylogenetic comparison with orthologous sequences from supposedly related extant genera from the Caprinae subfamily suggests that Myotragus is related to some of these species, however, the real phylogenetic position of MyotRAGus is difficult to assess, due to the lack of resolution of the present molecular study.

Extreme Endemic Radiation of the Malagasy Vangas (Aves: Passeriformes)

The results suggest the occurrence of an extensive in situ radiation of the Vangidae within Madagascar, and that the extant high diversity of this family is not the result of multiple colonizations from outside.

Molecular phylogenetic status of the iriomote cat Felis iriomotensis, inferred from mitochondrial DNA sequence analysis.

The results suggest that the geographic barrier has led the fixation of some unique morphological characters into the Iriomote cat population while both the Iris iriomotensis and the leopard cat still retain very close genetic characters.

A Molecular Test of Bat Relationships: Monophyly or Diphyly?

A phylogenetic analysis of 257 base pairs resulted in two shortest unrooted trees that significantly support the monophyly of the bats and also suggest that the colugo is more closely related to primates than to the bats.

Phylogenetic structure of the marsupial family dasyuridae based on cytochromeb DNA sequences

The objective in this study was to test the monophyly of Archer's seven groups and estimate relationships among them using DNA sequences from the mitochondrial cytochromeb (cyt-b) gene.



Albumin systematics of the extinct mammoth and Tasmanian wolf

The use of RIA to detect and characterize albumin in the soft tissues of two recently extinct species, the Siberian mammoth and the Tasmanian wolf, was found to be very similar to, and immunologically equidistant from, the albumins of the two living species of elephants.

Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers.

The polymerase chain reaction is used to amplify homologous segments of mtDNA from more than 100 animal species, including mammals, birds, amphibians, fishes, and some invertebrates, and the unexpectedly wide taxonomic utility of these primers offers opportunities for phylogenetic and population research.

Novel features of animal mtDNA evolution as shown by sequences of two rat cytochrome oxidase subunit II genes.

  • G. BrownM. Simpson
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1982
It is proposed that the rapid evolution of mtDNA relative to nuclear DNA is due only to silent changes and that amino acid-altering substitutions accumulate in nuclear and mtDNA at comparable rates.

Mitochondrial DNA sequences from a 7000-year old brain.

The sequences show that this ancient individual belonged to a mitochondrial lineage that is rare in the Old World and not previously known to exist among Native Americans, bringing to three the number of maternal lineages known to have been involved in the prehistoric colonization of the New World.

Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification.

  • S. Pääbo
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1989
The polymerase chain reaction can be used to amplify and study short mitochondrial DNA sequences that are of anthropological and evolutionary significance and opens up the prospect of performing diachronical studies of molecular evolutionary genetics.

Length mutations in human mitochondrial DNA: direct sequencing of enzymatically amplified DNA.

Phylogenetic analysis suggests that this deletion in region V occurred only once during the evolution of modern types of human mtDNA and that it will be a valuable anthropological marker for peoples of East Asian origin.


  • J. Felsenstein
  • Economics
    Evolution; international journal of organic evolution
  • 1985
The recently‐developed statistical method known as the “bootstrap” can be used to place confidence intervals on phylogenies and shows significant evidence for a group if it is defined by three or more characters.

Sequence and organization of the human mitochondrial genome

The complete sequence of the 16,569-base pair human mitochondrial genome is presented and shows extreme economy in that the genes have none or only a few noncoding bases between them, and in many cases the termination codons are not coded in the DNA but are created post-transcriptionally by polyadenylation of the mRNAs.

Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia. The first involves the primer-mediated enzymatic amplification of specific