DNA origami-based single-molecule force spectroscopy unravels the molecular basis of RNA Polymerase III pre-initiation complex stability

@inproceedings{Kramm2019DNAOS,
  title={DNA origami-based single-molecule force spectroscopy unravels the molecular basis of RNA Polymerase III pre-initiation complex stability},
  author={Kevin Kramm and Tim Schr{\"o}der and J{\'e}r{\^o}me Gouge and Andr{\'e}s Manuel Vera and Florian Heiss and Tim Liedl and Christoph Engel and Alessandro Vannini and Philip Tinnefeld and Dina Grohmann},
  year={2019}
}
Abstract The TATA-binding protein (TBP) and a transcription factor (TF) IIB-like factor compound the fundamental core of all eukaryotic initiation complexes. The reason for the emergence and strict requirement of the additional intiation factor Bdp1, which is unique to the RNA polymerase (RNAP) III sytem, however, remained elusive. A poorly studied aspect in this context is the effect of DNA strain, that arises from DNA compaction and transcriptional activity, on the efficiency of initiation… CONTINUE READING