DNA modification and functional delivery into human cells using Escherichia coli DH10B.

@article{Narayanan2003DNAMA,
  title={DNA modification and functional delivery into human cells using Escherichia coli DH10B.},
  author={Kumaran Narayanan and Peter E. Warburton},
  journal={Nucleic acids research},
  year={2003},
  volume={31 9},
  pages={
          e51
        }
}
  • Kumaran Narayanan, Peter E. Warburton
  • Published in Nucleic acids research 2003
  • Biology, Medicine
  • The availability of almost the complete human genome as cloned BAC libraries represents a valuable resource for functional genomic analysis, which, however, has been somewhat limited by the ability to modify and transfer this DNA into mammalian cells intact. Here we report a novel comprehensive Escherichia coli-based vector system for the modification, propagation and delivery of large human genomic BAC clones into mammalian cells. The GET recombination inducible homologous recombination system… CONTINUE READING

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