DNA methylation profiles in monozygotic and dizygotic twins

  title={DNA methylation profiles in monozygotic and dizygotic twins},
  author={Zachary A. Kaminsky and Thomas Tang and Sun-Chong Wang and Carolyn Ptak and Gabriel Oh and Albert Hung Choy Wong and Laura A. Feldcamp and Carl Virtanen and Jonas Halfvarson and Curt Tysk and Allan F. McRae and Peter M. Visscher and Grant W. Montgomery and Irving I Gottesman and Nicholas G. Martin and Artūras Petronis},
  journal={Nature Genetics},
Twin studies have provided the basis for genetic and epidemiological studies in human complex traits. As epigenetic factors can contribute to phenotypic outcomes, we conducted a DNA methylation analysis in white blood cells (WBC), buccal epithelial cells and gut biopsies of 114 monozygotic (MZ) twins as well as WBC and buccal epithelial cells of 80 dizygotic (DZ) twins using 12K CpG island microarrays. Here we provide the first annotation of epigenetic metastability of ∼6,000 unique genomic… 

DNA methylation variations between mono - and dizygotic twins

DNA methylation changes were studied in 16 MZ twin pairs and 8 dizygotic (DZ) twin pairs using CRED-RA technique and the most suitable primer (5’-GATGACCGCC-3’) was selected out of 20, random, decamer primers.

Genetic-epigenetic Interactions: Sequence-dependent and Independent DNA Nethylation

This study was the first to utilize epigenomewide profiling to document DNA methylation differences in MZ twins, and found that DZ twins exhibited more epigenetic differences compared to MZ siblings.

Differences of DNA methylation profiles between monozygotic twins’ blood samples

It is suggested that CpG methylation profile could be a useful biomarker in individual identification of MZs, which exhibited remarkable differences of genome-wide 5-methylcytosine.

Epigenetic Variation in Monozygotic Twins: A Genome-Wide Analysis of DNA Methylation in Buccal Cells

The findings indicate that it is worthwhile to examine heritable and shared environmental influences on buccal DNA methylation in larger studies that also include dizygotic twins, and suggests that individual-specific environmental and stochastic influences account for more variation in DNAmethylation in CpG-poor regions.

Intra-Monozygotic Twin Pair Discordance and Longitudinal Variation of Whole-Genome Scale DNA Methylation in Adults

It is demonstrated that, on whole-genome level, there has been no significant epigenetic drift within the same individuals for up to 9 months, including one monozygotic twin pair, and a subset of CpG sites that vary in DNA methylation over the 9-month period are identified.

Neonatal DNA methylation profile in human twins is specified by a complex interplay between intrauterine environmental and genetic factors, subject to tissue-specific influence.

This is the first study to analyze DNA methylation on a genome scale in twins at birth, further highlighting the importance of the intrauterine environment on shaping the neonatal epigenome and little evidence for genome-wide epigenetic drift with increasing age.

Genome-wide analysis of sperm DNA methylation from monozygotic twin bulls.

Differences in the sperm epigenome may contribute to incongruous diverging performances of daughters sired by bulls that are MZ twins.

DNA methylation analysis of multiple tissues from newborn twins reveals both genetic and intrauterine components to variation in the human neonatal epigenome.

The intrauterine period is confirmed as a sensitive time for the establishment of epigenetic variability in humans and an epigenetic mechanism for the previously described phenomenon of 'fetal programming' of disease risk is supported.

Epigenetics of discordant monozygotic twins: implications for disease

Advances in epigenetic studies of discordant MZ twins, focusing on disease, help to identify epigenetic markers of environmental risk and molecular mechanisms involved in disease and disease progression, which have implications both for understanding disease and for future medical research.



Monozygotic twins exhibit numerous epigenetic differences: clues to twin discordance?

Although the epigenetic analysis was conducted for only several hundred base pairs of DRD2, the fact that numerous studies identified nonuniform methylation patterns across the clones of bisulfite-modified DNA from the same individual, as well as non uniform patterns across different individuals, argues for the universality of intra- and interindividual epigenetic variation.

Epigenetic differences arise during the lifetime of monozygotic twins.

Older monozygous twins exhibited remarkable differences in their overall content and genomic distribution of 5-methylcytosine DNA and histone acetylation, affecting their gene-expression portrait, indicating how an appreciation of epigenetics is missing from the understanding of how different phenotypes can be originated from the same genotype.

Intra- and interindividual epigenetic variation in human germ cells.

Evidence for significant epigenetic variability in human germ cells is provided, which warrants further research to determine whether such epigenetic patterns can be efficiently transmitted across generations and what impact inherited epigenetic individuality may have on phenotypic outcomes in health and disease.

Aberrant DNA methylation associated with bipolar disorder identified from discordant monozygotic twins

The results suggest that altered DNA methylation statuses of PPIEL might have some significance in pathophysiology of bipolar disorder.

Phenotypically concordant and discordant monozygotic twins display different DNA copy-number-variation profiles.

Increased DNA methylation at the AXIN1 gene in a monozygotic twin from a pair discordant for a caudal duplication anomaly.

It is confirmed that this CpG island does function as a promoter in vitro and that its activity is inversely proportional to the extent of methylation, raising the possibility that hypermethylation of the AXIN1 promoter, by mechanisms as yet undetermined, is associated with the malformation.

Epigenomic profiling reveals DNA-methylation changes associated with major psychosis.

Genomic surveys by methylation-sensitive SNP analysis identify sequence-dependent allele-specific DNA methylation

Recurrent phenomenon of sequence-dependent ASM has practical implications for mapping and interpreting associations of noncoding SNPs and haplotypes with human phenotypes.

Phenotypic differences in genetically identical organisms: the epigenetic perspective.

Epigenetic mechanisms may explain paradoxical findings in twin and inbred animal studies when Phenotypic differences occur in the absence of observable environmental differences and also when environmental differences do not significantly increase the degree of phenotypic variation.

Comparative isoschizomer profiling of cytosine methylation: the HELP assay.

The HpaII tiny fragment Enrichment by Ligation-mediated PCR assay is robust, quantitative, and accurate and is providing new insights into the distribution and dynamic nature of cytosine methylation in the genome.