DNA methylation levels analysis in four tissues of sea cucumber Apostichopus japonicus based on fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP) during aestivation.

@article{Zhao2015DNAML,
  title={DNA methylation levels analysis in four tissues of sea cucumber Apostichopus japonicus based on fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP) during aestivation.},
  author={Ye Zhao and Muyan Chen and K. Storey and Lina Sun and Hongsheng Yang},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry \& molecular biology},
  year={2015},
  volume={181},
  pages={
          26-32
        }
}
  • Ye Zhao, Muyan Chen, +2 authors Hongsheng Yang
  • Published 2015
  • Biology, Medicine
  • Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
DNA methylation plays an important role in regulating transcriptional change in response to environmental stimuli. In the present study, DNA methylation levels of tissues of the sea cucumber Apostichopus japonicus were analyzed by the fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP) technique over three stages of the aestivation cycle. Overall, a total of 26,963 fragments were amplified including 9112 methylated fragments among four sea cucumber tissues using 18 pairs… Expand
Genome-wide analysis of DNA methylation in five tissues of sika deer (Cervus nippon).
TLDR
This study presents the first look at the T-DMRs in sika deer and represents an initial step towards understanding of epigenetic regulatory mechanism underlying tissue development and differentiation in sIKA deer. Expand
Analysis of DNA methylation level by methylation-sensitive amplification polymorphism in half smooth tongue sole (Cynoglossus semilaevis) subjected to salinity stress
TLDR
DNA methylation regulates tissue- specific epiloci, and may respond to salinity stress by regulating gene expression to maintain animal survival and activity, as a result of regulation of epigenetic mechanism in environmental stimuli. Expand
Gene structure, expression, and DNA methylation characteristics of sea cucumber cyclin B gene during aestivation.
TLDR
It is suggested that cell cycle progression may be reversibly arrested during aestivation as indicated by the changes in cyclin B expression levels and it is proposed that DNA methylation is one of the regulatory mechanisms involved in Cyclin B transcriptional variation. Expand
Changes of cytosine methylation in pecan tissues of different stages by quantitative methylation-sensitive amplified polymorphism
TLDR
The patterns of methylation variation and methylation relationships among pecan tissues undergoing different developmental processes are revealed, implying the important roles ofmethylation in tissue differentiation and development of trees. Expand
Analysis of DNA Methylation Level of Portunus trituberculatus Subjected to Low Salinity with Methylation-Sensitive Amplification Polymorphism
TLDR
The results indicated that DNA methylation is tissue-specific when P. trituberculatus responds to low salinity, and both demethylation and methylation processes occurred under low Salinity stress. Expand
Identification and sequencing of the gene encoding DNA methyltransferase 3 (DNMT3) from sea cucumber, Apostichopus japonicus
TLDR
The newly sequencedDNMT3 gene comprises three major conserved domains, indicating that the DNMT3 possibly has de novo DNA methylation catalytic activity, and may provide insights into the sea cucumber biology. Expand
Genome-wide DNA methylation analysis of the regenerative and non-regenerative tissues in sika deer (Cervus nippon).
TLDR
The data suggest that DNA methylation, an important epigenetic regulation mechanism, may play an important role in the mammalian tissue/organ regeneration. Expand
Analysis of Genomewide DNA Methylation Reveals Differences in DNA Methylation Levels between Dormant and Naturally as well as Artificially Potentiated Pedicle Periosteum of Sika Deer (Cervus nippon).
TLDR
The data suggest that DNA demethylation is involved in the process of PP potentiation, which is a prerequisite step for the initiation of antler regeneration, and provides the first experimental evidence to link epigenetic regulation and mammalian appendage regeneration. Expand
Effects of temperature and salinity on survival, growth and DNA methylation of juvenile Pacific abalone, Haliotis discus hannai Ino
TLDR
The results of this study provide optimal rearing conditions for juvenile H. discus hannai Ino, and represent the first step toward revealing the epigenetic regulatory mechanism of abalone in response to thermal and salt stresses. Expand
Glycolytic regulation in aestivation of the sea cucumber Apostichopus japonicus: evidence from metabolite quantification and rate-limiting enzyme analyses
TLDR
Results suggest that glycolytic suppression occurred in aestivating A. japonicus, especially in the intestine, and transcriptional and post-transcriptional modifications are involved in the regulation of glycoleytic suppression in this marine species. Expand
...
1
2
3
...

References

SHOWING 1-10 OF 58 REFERENCES
Molecular cloning and expression-profile analysis of sea cucumber DNA (cytosine-5)-methyltransferase 1 and methyl-CpG binding domain type 2/3 genes during aestivation.
TLDR
The results of this study suggest that DNA methylation may be involved in transcriptional silencing, and that the intestine is the major site for epigenetic regulation during aestivation in the sea cucumber. Expand
Analysis of the DNA methylation of maize (Zea mays L.) in response to cold stress based on methylation-sensitive amplified polymorphisms
TLDR
The results may indicate that the specific demethylation of genes is an active and rapid epigenetic response to cold in maize during the seedling stage, further elucidating the mechanism of maize adaptation to cold stress. Expand
MSAP ANALYSIS OF GENOME DNA METHYLATION IN DIFFERENT TISSUES OF APOSTICHOPUS JAPONICUS
TLDR
The statistic results showed that the intestine had significant difference with the other three tissues, and the difference of methylation between muscle,respiratory tree and body wall was not obvious, while there was significant difference between muscle and respiration tree in hemi-methylation. Expand
Analysis of the methylation in genome DNA from different tissues of Anodonta woodiana
TLDR
The comparison of the swan mussels from different habitats suggested there were some variations in methylated gene loci between those from polluted environment and aquacultural water (Nanquan aquaculture base of Freshwater Fisheries Research Centre, Chinese Academy of Fishery Sciences). Expand
Analysis of DNA methylation related to rice adult plant resistance to bacterial blight based on methylation-sensitive AFLP (MSAP) analysis
TLDR
The results suggest that significant differences in cytosine methylation exist between seedlings and adult plants, and that hypermethylation or hypomethylation of specific genes may be involved in the development of adult plant resistance (APR) in rice plants. Expand
Genome-Wide Analysis of DNA Methylation in Five Tissues of Zhikong Scallop, Chlamys farreri
TLDR
This study presents the first look at the tissue-specific DNA methylation patterns in a bivalve mollusc and represents an initial step towards understanding of epigenetic regulatory mechanism underlying tissue development and differentiation in bivalves. Expand
Patterns of cytosine methylation in an elite rice hybrid and its parental lines, detected by a methylation-sensitive amplification polymorphism technique
TLDR
The results clearly demonstrate that the MSAP technique is highly efficient for large-scale detection of cytosine methylation in the rice genome and believe that the technique can be adapted for use in other plant species. Expand
Analysis of DNA Methylation in Various Swine Tissues
TLDR
The results clearly demonstrated that the F-MSAP technique can be adapted for use in large-scale DNA methylation detection in the pig genome. Expand
Transcriptional changes in epigenetic modifiers associated with gene silencing in the intestine of the sea cucumber, Apostichopus japonicus (Selenka), during aestivation
TLDR
The results suggest that the expression of epigenetic modifiers involved in DNA methylation, chromatin remodeling, histone acetylation, and histone methylation is upregulated during aestivation. Expand
Analysis on DNA Methylation of Various Tissues in Chicken
TLDR
The genome-wide DNA methylation patterns of CCGG sites were detected with fluorescent-labeled methylation sensitive amplified polymorphism (F-MSAP) in three chicken strains, implying that such differences may be related to gene expression during tissue differentiation, growth, and development though the relation between methylation and gene expression is currently hard to assess. Expand
...
1
2
3
4
5
...