DNA methylation is related to the occurrence of breast cancer and is not affected by culture conditions.

@article{Wang2018DNAMI,
  title={DNA methylation is related to the occurrence of breast cancer and is not affected by culture conditions.},
  author={Shibao Wang and Yinghui Huang and Xupeng Mu and Tianyang Qi and Sha Qiao and Zhenxia Lu and Hong-jun Li},
  journal={Molecular medicine reports},
  year={2018},
  volume={17 5},
  pages={
          7365-7371
        }
}
The present study aimed to explore the relationship between DNA methylation and breast cancer under different cell culture conditions. MCF‑7 breast cancer cells were cultured in two‑dimensional (2D), three‑dimensional (3D) and orthotopic transplantation (Ti) adhesion substrates. Principal component analysis (PCA) was used for global visualization of these three samples. The methylation status of CpG sites was examined by unsupervised clustering analysis. Scatter plots and histograms were… 
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