DNA metabarcoding of insects and allies: an evaluation of primers and pipelines.

  title={DNA metabarcoding of insects and allies: an evaluation of primers and pipelines.},
  author={G-J Brandon-Mong and H Y Gan and K W Sing and P. Lee and P E Lim and J-J Wilson},
  journal={Bulletin of entomological research},
  volume={105 6},
Metabarcoding, the coupling of DNA-based species identification and high-throughput sequencing, offers enormous promise for arthropod biodiversity studies but factors such as cost, speed and ease-of-use of bioinformatic pipelines, crucial for making the leapt from demonstration studies to a real-world application, have not yet been adequately addressed. Here, four published and one newly designed primer sets were tested across a diverse set of 80 arthropod species, representing 11 orders, to… CONTINUE READING
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