DNA melting investigated by differential scanning calorimetry and Raman spectroscopy.

@article{Duguid1996DNAMI,
  title={DNA melting investigated by differential scanning calorimetry and Raman spectroscopy.},
  author={John G. Duguid and Victor A. Bloomfield and James M. Benevides and George J. Thomas},
  journal={Biophysical journal},
  year={1996},
  volume={71 6},
  pages={3350-60}
}
Thermal denaturation of the B form of double-stranded DNA has been probed by differential scanning calorimetry (DSC) and Raman spectroscopy of 160 base pair (bp) fragments of calf thymus DNA. The DSC results indicate a median melting temperature Tm = 75.5 degrees C with calorimetric enthalpy change delta Hcal = 6.7 kcal/mol (bp), van't Hoff enthalpy change delta HVH = 50.4 kcal/mol (cooperative unit), and calorimetric entropy change delta Scal = 19.3 cal/deg.mol (bp), at the experimental… CONTINUE READING

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