DNA looping kinetics analyzed using diffusive hidden Markov model.

@article{Beausang2007DNALK,
  title={DNA looping kinetics analyzed using diffusive hidden Markov model.},
  author={John F. Beausang and Chiara Zurla and Carlo Manzo and David Dunlap and Laura Finzi and Philip C. Nelson},
  journal={Biophysical journal},
  year={2007},
  volume={92 8},
  pages={L64-6}
}
Tethered particle experiments use light microscopy to measure the position of a micrometer-sized bead tethered to a microscope slide via an approximately micrometer-length polymer, to infer the behavior of the invisible polymer. Currently, this method is used to measure rate constants of DNA loop formation and breakdown mediated by repressor protein that binds to the DNA. We report a new technique for measuring these rates using a modified hidden Markov analysis that directly incorporates the… CONTINUE READING