DNA-linked Inhibitor Antibody Assay (DIANA) for sensitive and selective enzyme detection and inhibitor screening

@inproceedings{Navratil2017DNAlinkedIA,
  title={DNA-linked Inhibitor Antibody Assay (DIANA) for sensitive and selective enzyme detection and inhibitor screening},
  author={Vaclav Navratil and Jiř{\'i} Schimer and Jan Tykvart and Tom{\'a}{\vs} Knedl{\'i}k and Viktor Vik and Pavel Majer and Jan Konvalinka and Pavel {\vS}{\'a}cha},
  booktitle={Nucleic acids research},
  year={2017}
}
Human diseases are often diagnosed by determining levels of relevant enzymes and treated by enzyme inhibitors. We describe an assay suitable for both ultrasensitive enzyme quantification and quantitative inhibitor screening with unpurified enzymes. In the DNA-linked Inhibitor ANtibody Assay (DIANA), the target enzyme is captured by an immobilized antibody, probed with a small-molecule inhibitor attached to a reporter DNA and detected by quantitative PCR. We validate the approach using the… CONTINUE READING

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SHOWING 1-10 OF 50 REFERENCES

Carborane-based carbonic anhydrase inhibitors.

  • Angewandte Chemie
  • 2013
VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL