DNA ligase and the pyridine nucleotide cycle in Salmonella typhimurium.

  title={DNA ligase and the pyridine nucleotide cycle in Salmonella typhimurium.},
  author={U E Park and Baldomero M Olivera and Kelly T Hughes and John R. Roth and David R. Hillyard},
  journal={Journal of bacteriology},
  volume={171 4},
Bacterial DNA ligases use NAD as an energy source. In this study we addressed two questions about these enzymes. First, what is the physiological consequence of completely removing the NAD-dependent enzyme and replacing it with an ATP-dependent DNA ligase? We constructed Salmonella typhimurium strains in which the endogenous NAD-dependent DNA ligase activity was inactivated by an insertion mutation and the ATP-dependent enzyme from bacteriophage T4 was provided by a cloned phage gene. Such… CONTINUE READING


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