DNA ligase I mediates essential functions in mammalian cells.

  title={DNA ligase I mediates essential functions in mammalian cells.},
  author={John H.J Petrini and Yang Xiao and David T. Weaver},
  journal={Molecular and cellular biology},
  volume={15 8},
DNA replication, repair, and recombination are essential processes in mammalian cells. Hence, the application of gene targeting to the study of these DNA metabolic pathways requires the creation of nonnull mutations. We have developed a method for introducing partially defective mutants in murine embryonic stem cells that circumvents the problem of cellular lethality of targeted mutations at essential loci. Using this approach, we have determined that mammalian DNA ligase I is essential for… CONTINUE READING

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