DNA large restriction fragment patterns of sporadic and epidemic nosocomial strains of Mycobacterium chelonae and Mycobacterium abscessus.

@article{Wallace1993DNALR,
  title={DNA large restriction fragment patterns of sporadic and epidemic nosocomial strains of Mycobacterium chelonae and Mycobacterium abscessus.},
  author={Richard J. Wallace and Yansheng Zhang and Barbara A Brown and Victoria F Fraser and Gerald H Mazurek and S. B. Maloney},
  journal={Journal of clinical microbiology},
  year={1993},
  volume={31 10},
  pages={2697-701}
}
Large restriction fragment (LRF) pattern analysis of genomic DNA using pulsed-field gel electrophoresis was performed on three reference strains, 32 sporadic isolates, and 92 nosocomial isolates from 12 epidemics of Mycobacterium chelonae and Mycobacterium abscessus. Only 17 of 30 (57%) unrelated strains of M. abscessus, compared with 10 of 11 (91%) of M. chelonae strains, gave satisfactory DNA extractions, with the remainder resulting in highly fragmented DNA. DraI, AsnI, XbaI, and SpeI gave… CONTINUE READING
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