DNA labeling in living cells.

@article{Martin2005DNALI,
  title={DNA labeling in living cells.},
  author={Robert M. Martin and Heinrich Leonhardt and M Cristina Cardoso},
  journal={Cytometry. Part A : the journal of the International Society for Analytical Cytology},
  year={2005},
  volume={67 1},
  pages={45-52}
}
BACKGROUND Live cell fluorescence microscopy experiments often require visualization of the nucleus and the chromatin to determine the nuclear morphology or the localization of nuclear compartments. METHODS We compared five different DNA dyes, TOPRO-3, TOTO-3, propidium iodide, Hoechst 33258, and DRAQ5, to test their usefulness in live cell experiments with continuous imaging and photobleaching in widefield epifluorescence and confocal laser scanning microscopy. In addition, we compared the… CONTINUE READING
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