DNA integrity in sexed bull sperm assessed by neutral Comet assay and sperm chromatin structure assay.

@article{BoeHansen2005DNAII,
  title={DNA integrity in sexed bull sperm assessed by neutral Comet assay and sperm chromatin structure assay.},
  author={G B Boe-Hansen and Ian D. Morris and Annette Kjaer Ersb{\o}ll and Torben Greve and Preben Christensen},
  journal={Theriogenology},
  year={2005},
  volume={63 6},
  pages={
          1789-802
        }
}
During the production of sex-sorted spermatozoa from bull semen, the cells are exposed to a number of potential hazards including: dilution, centrifugation, incubation, exposure to DNA stains and laser light. These factors may affect the survival capacity and fertilization potential of the sperm. The objective of this study was to determine whether sex-sorted bull spermatozoa have more DNA damage than sperm from conventional processed bull semen. Two methods were used to determine DNA integrity… CONTINUE READING

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