DNA integrity and motility of human spermatozoa after standard slow freezing versus cryoprotectant-free vitrification.

@article{Isachenko2004DNAIA,
  title={DNA integrity and motility of human spermatozoa after standard slow freezing versus cryoprotectant-free vitrification.},
  author={Eugenia Isachenko and Vladimir Isachenko and Igor I. Katkov and G. K. Mahdi Rahimi and Thomas Sch{\"o}ndorf and Peter K Mallmann and Salvatore Dessole and Frank Nawroth},
  journal={Human reproduction},
  year={2004},
  volume={19 4},
  pages={932-9}
}
BACKGROUND In contrast to the technique of conventional freezing, the vitrification of spermatozoa requires high cooling rates (720 000 degrees K/min), which could be damaging for spermatozoa. The aim of our study was to compare slowly frozen and vitrified spermatozoa in terms of their post-thaw DNA integrity and motility. METHODS Semen samples were prepared according to the routine swim-up technique and divided into aliquots for comparison of fresh, conventionally frozen and vitrified… CONTINUE READING

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