DNA injection into single cells of intact mice.

  title={DNA injection into single cells of intact mice.},
  author={Jo Kristian Utvik and Arild Nj{\aa} and Kristian Kroyer Gundersen},
  journal={Human gene therapy},
  volume={10 2},
We describe a technique wherein single muscle fibers of intact mice are made transgenic by intracellular injection of DNA expression vectors for the reporter genes lacZ and green fluorescent protein (GFP). Application of in vivo imaging techniques allowed identification of single cells during the injections, and of the same single cells when the muscle was reexposed days or weeks later. DNA concentration by itself had little effect on fiber survival or expression efficacy, but it seemed crucial… CONTINUE READING

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