DNA in situ sensitivity to denaturation in bladder cancer and its correlation with tumor stage.

@article{Bretton1990DNAIS,
  title={DNA in situ sensitivity to denaturation in bladder cancer and its correlation with tumor stage.},
  author={Philippe Bretton and Zbigniew Darzynkiewicz and Edgar Henry and Melanie Kimmel and William R. Fair and Myron R. Melamed},
  journal={Cancer research},
  year={1990},
  volume={50 24},
  pages={
          7912-4
        }
}
DNA content and sensitivity of DNA in situ to denaturation by acid were analyzed by flow cytometry of cell nuclei freshly isolated from the bladder tumors of 32 patients and were compared with normal urothelium of 8 subjects. DNA sensitivity to denaturation was assessed in RNase treated cells by acridine orange metachromasia following partial denaturation with hydrochloric acid; the extent of denatured DNA is given as an index (alpha t), representing the ratio of single stranded to total DNA… CONTINUE READING

References

Publications referenced by this paper.
SHOWING 1-10 OF 19 REFERENCES

Flow cytometry in surgical pathology and cytology of the genitourinary tract

B. Tribukait
  • Advances in Clinical Cytology,
  • 1984
VIEW 1 EXCERPT