DNA in situ hybridization on polytene chromosomes of Simulium sanctipauli at loci relevant to insecticide resistance.

@article{Boakye2000DNAIS,
  title={DNA in situ hybridization on polytene chromosomes of Simulium sanctipauli at loci relevant to insecticide resistance.},
  author={Daniel Adjei Boakye and Anthony J. Cornel and S. E. O. Meredith and Paul Martin Brakefield and Frank H. Collins},
  journal={Medical and veterinary entomology},
  year={2000},
  volume={14 2},
  pages={217-22}
}
A DNA technique for in situ hybridization developed by Kumar & Collins (1994) for use on polytene chromosomes of adult Anopheles mosquitoes (Diptera: Culicidae) was modified for use with Simulium larval salivary gland chromosomes (Diptera: Simuliidae). Cloned fragments of several Simulium genes (coding for aspartate amino transferase, cytochrome P450 and DNA polymerase) were successfully mapped physically by assigning specific band locations in Simulim sanctipauli V. & D. This represents the… CONTINUE READING