DNA-free liposome-assisted transfection of Streptococcus faecium SF 77R protoplasts.

Abstract

Two virulent phages of Streptococcus faecium SF 77R were used in DNA-free liposome assisted polyethylene-glycol-mediated protoplast transfection. This procedure, established to develop an effective gene-cloning system, optimized the conditions for DNA uptake by the protoplasts of Str. faecium. Transfection frequencies of up to 10(7)-10(8) P.F.U./microgram… (More)

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