DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

@article{Woo2015DNAfreeGE,
  title={DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins},
  author={Je Wook Woo and Jungeun Kim and Soon Il Kwon and Claudia Corval{\'a}n and Seung Woo Cho and HyeRan Kim and Sang-Gyu Kim and Sang-Tae Kim and Sunghwa Choe and Jin-Soo Kim},
  journal={Nature Biotechnology},
  year={2015},
  volume={33},
  pages={1162-1164}
}
Editing plant genomes without introducing foreign DNA into cells may alleviate regulatory concerns related to genetically modified plants. We transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%. The targeted sites contained germline-transmissible small insertions or deletions that are indistinguishable from naturally… CONTINUE READING
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