DNA fragmentation of oocytes in aged mice.

@article{Fujino1996DNAFO,
  title={DNA fragmentation of oocytes in aged mice.},
  author={Yoko Fujino and Koichi Ozaki and Seiichi Yamamasu and Fusahiko Ito and Ichiro Matsuoka and Emiko Hayashi and Hitomi Nakamura and Sachio Ogita and Eimi Sato and Masaki Kitakyushu-shi Inoue},
  journal={Human reproduction},
  year={1996},
  volume={11 7},
  pages={
          1480-3
        }
}
To investigate whether female fertility decreases with age due to poor oocyte quality, we examined the presence of DNA fragmentation in ovulated oocytes from young, mature and aged mice. Oocytes from three age groups of female mice (7-8, 20-24 and 40-48 weeks) were retrieved from the oviducts 15 h after human chorionic gonadotrophin (HCG) injection. Oocytes from each mouse were incubated in a CO2 incubator for 0-60 h in human tubal fluid (HTF). After incubation, each oocyte was stained with the… CONTINUE READING

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