DNA fragmentation in mammalian cells exposed to various light ions.

@article{Belli2001DNAFI,
  title={DNA fragmentation in mammalian cells exposed to various light ions.},
  author={M. Belli and R. Cherubini and M. Dalla Vecchia and V. Dini and G. Esposito and G. Moschini and O. Sapora and C. Signoretti and G. Simone and E. Sorrentino and M. Tabocchini},
  journal={Advances in space research : the official journal of the Committee on Space Research},
  year={2001},
  volume={27 2},
  pages={
          393-9
        }
}
  • M. Belli, R. Cherubini, +8 authors M. Tabocchini
  • Published 2001
  • Chemistry, Medicine
  • Advances in space research : the official journal of the Committee on Space Research
Elucidation of how effects of densely ionizing radiation at cellular level are linked to DNA damage is fundamental for a better understanding of the mechanisms leading to genomic damage (especially chromosome aberrations) and developing biophysical models to predict space radiation effects. We have investigated the DNA fragmentation patterns induced in Chinese hamster V79 cells by 31 keV/micrometer protons, 123 keV/micrometer helium-4 ions and gamma rays in the size range 0.023-5.7 Mbp, using… Expand
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Accounting for radiation-induced indirect damage on DNA with the Geant 4-DNA code.
TLDR
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References

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DNA double strand break production and rejoining in V79 cells irradiated with light ions.
  • M. Belli, F. Ianzini, +7 authors G. Simone
  • Physics, Medicine
  • Advances in space research : the official journal of the Committee on Space Research
  • 1996
TLDR
The results indicate that dsb are not homogeneous with respect to repair and give support to the hypothesis that increasing LET leads to an increase in the complexity of DNA lesions with a consequent decrease in their repairability. Expand
DNA double-strand break distributions in X-ray and alpha-particle irradiated V79 cells: evidence for non-random breakage.
TLDR
This study shows that the distribution of damage from a high LET alpha-particle track is significantly different from that observed with low LET X-rays, and suggests that the fragmentation patterns of irradiated DNA may be related to the higher-order chromatin repeating structures found in intact cells. Expand
DNA double-strand breaks induced by low energy protons in V79 cells.
TLDR
The initial production of DNA double-strand breaks (dsb) was determined in V79 Chinese hamster cells irradiated with proton beams and no significant differences for dsb induction were found between protons and X-rays. Expand
Induction of DNA double-strand breaks in CHO-K1 cells by carbon ions.
TLDR
Radiation-induced DNA double-strand breaks were measured in CHO-K1 cells by means of an experimental approach involving constant-field gel electrophoresis and densitometric scanning of ethidium bromide stained gels, compared with published results on dsb induction in mammalian cells by radiations of comparable LET. Expand
Non-random distribution of DNA double-strand breaks induced by particle irradiation.
TLDR
The total induction rate of dsbs for the three radiations is determined based on a quantitative analysis of all the measured radiation-induced fragments and it is found that the high LET particles were more efficient than X-rays at inducing dsBS, indicating an increasing total efficiency with LET. Expand
Simulation of DNA fragment distributions after irradiation with photons
TLDR
Simulations of the model of the genome inside a human cell nucleus in its interphase based on the atomic coordinates of the DNA double helix with an additional volume for the water shell show improvements in electron scattering cross-sections for liquid water and the interaction of water radicals with DNA. Expand
DNA DSB induction and rejoining in V79 cells irradiated with light ions: a constant field gel electrophoresis study.
TLDR
The hypothesis that complex, less reparable DSB are induced in higher proportion by light ions with respect to gamma-rays and that, for the same ion, increasing LET leads to an increase in this proportion is supported. Expand
Chromatin conformation in living cells: support for a zig-zag model of the 30 nm chromatin fiber.
TLDR
Comparison of the experimental data with theoretical results support a zig-zag model of the chromatin fiber rather than a simple helical model, and radiation-induced damage analysis can provide information on chromatin structure in the living cell. Expand
A quantitative model of DNA fragments generated by ionizing radiation, and possible experimental applications.
TLDR
An equation for observed frequencies of DNA fragments as a function of size is derived, which is consistent with experimental and theoretical results reported elsewhere and proposes that similar experiments might be suitable for precise measurements of the parameters of double-strand breakage and for testing the assumption of random, independent breakage for different types of radiation. Expand
Quantification of DNA damage by PFGE: development of an analytical approach to correct for the background distribution.
TLDR
An alternative approach based on correcting the fitting functions for the actual nonrandom damage present in the control unirradiated samples has been developed. Expand
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