DNA fluorescence induced by polymethine cation pyrvinium binding

  title={DNA fluorescence induced by polymethine cation pyrvinium binding},
  author={Juan Carlos Stockert and Clara Isabel Trigoso and Alicia Llorente and Pablo Castillo},
  journal={The Histochemical Journal},
Pyrvinium is a polymethine cation which shows interesting fluorescence emission and DNA binding properties. In diluted aqueous solution, pyrvinium pamoate induced a bright yellow fluorescence in kinetoplast DNA fromTrypanosoma cruzi epimastigotes as well as in chicken erythrocyte nuclei under a wide range of excitations. No fading was observed after mounting in suitable media. Spectroscopic studies on pyrvinium solutions revealed bathochromic and hypochromic shifts in the absorption spectrum of… CONTINUE READING