DNA fingerprinting reveals clonal nature of Vetiveria zizanioides (L.) Nash, Gramineae and sources of potential new germplasm

  title={DNA fingerprinting reveals clonal nature of Vetiveria zizanioides (L.) Nash, Gramineae and sources of potential new germplasm},
  author={R. Adams and M. Z. Y. Turuspekov and M. R. Dafforn and J. Veldkamp},
  journal={Molecular Ecology},
Random amplified polymorphic DNAs (RAPDs) were used to examine accessions of vetiver (Vetiveria zizanioides (L.) Nash, Gramineae) from around the world. It appears that only one genotype, ‘Sunshine’, accounts for almost all the germplasm utilized. Screening of numerous accessions of vetiver revealed only a few nonfertile germlines. Analysis of V. zizanioides and other Vetiveria species, Chrysopogon fulvus (Spreng.) Chiov., C. gryllus (L.) Trin., Sorghum bicolour (L.) Moench., and S. halepense… Expand
Genetic diversity of vetiver isolates (Chrysopogon zizanioides/nigritanus) available in South Africa based on ITS, ndhF and rbcL sequencing analyses
Very little or no genotypical differences exist amongst the different isolates available from the Hydromulch (Pty) Ltd plant collection, and only in the case of the ITS inference were differences observed between 3 isolates. Expand
Complete chloroplast genome sequencing of vetiver grass (Chrysopogon zizanioides) identifies markers that distinguish the non-fertile ‘Sunshine’ cultivar from other accessions
Variations in cp genome sequence can be used to assess sterility in other accessions through sequencing or by the newly developed CAPS assay to uniquely identify Sunshine genomes. Expand
Molecular and chemical characterization of vetiver, Chrysopogon zizanioides (L.) Roberty, germplasm.
The molecular characteristics of the accessions were studied using amplified fragment length polymorphism markers, with a total of 14 primer combinations that generated 442 loci, allowing to observe that these accessions have similar genomes. Expand
Molecular diversity and SSR transferability studies in Vetiver grass (Vetiveria zizanioides L. Nash)
Analysis of molecular variance (AMOVA) indicated that 3% diversity was attributed to differences among regions, 1% at the level of populations and 96% contributed by the individuals in the populations. Expand
Vetiver DNA-Fingerprinted Cultivars: Effects of Environment on Growth, Oil Yields and Composition
Twenty one accessions of vetiver and Khus were analyzed by the use of random amplified polymorphic DNAs and no single genotype grew best in every plot. Expand
Characterisation of germplasm accessions of Napier grass (pennisetum purpureum and P. purpureum×P. glaucum Hybrids) and comparison with farm clones using RAPD
Comparison of intra and inter-accession variation identified several groups of identical/similar accessions that could be targeted if the collection is to be rationalised, and also highlighted two misplantings of germplasm material during transfer to a field trial site. Expand
Composition and functional comparison of vetiver root endophytic microbiota originating from different geographic locations that show antagonistic activity towards Fusarium graminearum.
The results showed that geographical location is a factor that influences the composition and relative abundance of root endophyte communities in vetiver, and the composition of these functional culturable endophytic communities was influenced by the geographic origins of the roots. Expand
Comparison of vetiver root essential oils from cleansed (bacteria- and fungus-free) vs. non-cleansed (normal) vetiver plants
Statistics revealed numerous significant differences between tissue culture derived and natural plants for both genotypes, and the thesis that many of the compounds found in vetiver roots originate from endogenous fungi was not supported. Expand
De novo assembly and characterization of root transcriptome in two distinct morphotypes of vetiver, Chrysopogon zizaniodes (L.) Roberty
The present study provided an important start point for further discovery of genes related to root oil quality in different ecotypes of vetiver. Expand
Genetic diversity of Vetiver clones (Chrysopogon zizanioides and Chrysopogon nigritana) available in South Africa based on sequencing analyses and anatomical structure
Vetiver grass or Chrysopogon zizanioides (L.) Roberty (1960) is a sterile grass which can regenerate vegetatively from clumps of the rootstock, which makes it an ideal candidate for the use in soil remediation and erosion control. Expand


Applications of Molecular Methods and Statistical Analyses for Discrimination of Accessions and Clones of Vetiver Grass
The authors' ability to generate consistent results may have been a result of genomic DNA quality, rigorous oligonucleotide primer selection, and DNA amplification protocol stringency. Expand
Systematic relationships in Juniperus based on random amplified polymorphic DNAs (RAPDs)
44 taxa of Juniperus were analysed using RAPDs, similarity measures and principal coordinate analyses (PCO) to confirm earlier taxonomic work based on leaf terpenoids and find the genus appears to be naturally divided into three major sections. Expand
Drierite and silica gel were found to be equally effective in preserving DNA in spinach leaves for up to 6 months at 37°C, which appears to be an acceptable method for the preservation of DNA in tropical plant leaf specimens for subsequent extraction and archiving or analyses. Expand
A rapid DNA isolation procedure for small quantities of fresh leaf tissue
Abstract A stopped flow kinetic analysis has been performed with a homogeneous protein fraction of plant glutamate dehydrogenase. The enzyme exerts strong negative cooperativity with ammonium asExpand
Some distance properties of latent root and vector methods used in multivariate analysis
SUMMARY This paper is concerned with the representation of a multivariate sample of size n as points P1, P2, ..., PI in a Euclidean space. The interpretation of the distance A(Pi, Pj) between the ithExpand
Gene banks and the world’s food
Feel lonely? What about reading books? Book is one of the greatest friends to accompany while in your lonely time. When you have no friends and activities somewhere and sometimes, reading book can beExpand
A General Coefficient of Similarity and Some of Its Properties
A general coefficient measuring the similarity between two sampling units is defined. The matrix of similarities between all pairs of sample units is shown to be positive semidefinite (exceptExpand
Preservation of DNA in plant specimens from endangered tropical species by desiccation
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