DNA fingerprinting of Streptococcus pneumoniae strains by pulsed-field gel electrophoresis.

@article{Lefevre1993DNAFO,
  title={DNA fingerprinting of Streptococcus pneumoniae strains by pulsed-field gel electrophoresis.},
  author={Jonathan Lefevre and G{\`e}nevi{\'e}ve Faucon and Armand Michel Sicard and Anne M E Gasc},
  journal={Journal of clinical microbiology},
  year={1993},
  volume={31 10},
  pages={2724-8}
}
Pulsed-field gel electrophoresis of genomic DNA was carried out on Streptococcus pneumoniae strains to determine its value in the epidemiological survey of pneumococcal infections. Twenty-one clinical strains were chosen to cover a broad range of diversity according to geographic location, penicillin susceptibility, serotype, and multilocus enzyme electrophoresis (MLEE) pattern. The restriction endonucleases ApaI and SmaI were used to digest intact chromosomes, and the fragments were resolved… CONTINUE READING

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