DNA fingerprinting of Escherichia coli O157:H7 strains by pulsed-field gel electrophoresis.

@article{Bhm1992DNAFO,
  title={DNA fingerprinting of Escherichia coli O157:H7 strains by pulsed-field gel electrophoresis.},
  author={H. W. B{\"o}hm and Helge Karch},
  journal={Journal of clinical microbiology},
  year={1992},
  volume={30 8},
  pages={2169-72}
}
Pulsed-field gel electrophoresis of genomic DNA was carried out on Escherichia coli O157:H7 strains from different geographic locations to determine its value in an epidemiological survey of O157 infections. Pulsed-field gel electrophoresis of XbaI-digested DNA fragments clearly separated E. coli O157:H7 strains from nontoxigenic E. coli O157:H19, O157:H43, and O157:H45 strains and from Shiga-like-toxin-producing E. coli strains of other serogroups. However, among the E. coli O157:H7 strains… CONTINUE READING