DNA family shuffling of hyperthermostable beta-glycosidases.

  title={DNA family shuffling of hyperthermostable beta-glycosidases.},
  author={Thijs Kaper and Stan J J Brouns and A. Max Geerling and Willem M de Vos and John van der Oost},
  journal={The Biochemical journal},
  volume={368 Pt 2},
The structural compatibility of two hyperthermostable family 1 glycoside hydrolases, Pyrococcus furiosus CelB and Sulfolobus solfataricus LacS, as well as their kinetic potential were studied by construction of a library of 2048 hybrid beta-glycosidases using DNA family shuffling. The hybrids were tested for their thermostability, ability to hydrolyse lactose and sensitivity towards inhibition by glucose. Three screening rounds at 70 degrees C led to the isolation of three high-performance… CONTINUE READING