DNA extraction procedure: a critical issue for bacterial diversity assessment in marine sediments.

@article{Luna2006DNAEP,
  title={DNA extraction procedure: a critical issue for bacterial diversity assessment in marine sediments.},
  author={Gian Marco Luna and Antonio Dell'anno and Roberto Danovaro},
  journal={Environmental microbiology},
  year={2006},
  volume={8 2},
  pages={308-20}
}
In order to evaluate whether different DNA extraction procedures can affect estimates of benthic bacterial diversity, based on 16S rRNA gene terminal restriction fragment length polymorphism (T-RFLP) fingerprinting technique, we compared two in situ lysis procedures (a SDS-based protocol and a commercial kit for DNA recovery) and one cell-extraction protocol on a variety of marine sediments. Despite the two in situ lysis procedures resulted in significantly different DNA yields (highest with… CONTINUE READING

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