DNA extraction from spider webs

@article{Blake2016DNAEF,
  title={DNA extraction from spider webs},
  author={Max Blake and Niall J. McKeown and M Bushell and Paul W. Shaw},
  journal={Conservation Genetics Resources},
  year={2016},
  volume={8},
  pages={219-221}
}
Many spider species produce webs that represent a potential non-invasive source of DNA for conservation genetic analysis. Reported here is the successful isolation of target DNA from members of two families (Theraphosidae and Pholcidae) using a standard CTAB phenol–chloroform–isoamyl protocol. The isolated DNA was of sufficient quality to permit routine PCR amplification and sequencing of mtDNA COI fragments of various sizes (maximum 710 bp attempted). This adds to other studies in… 

Spider webs as eDNA samplers: Biodiversity assessment across the tree of life

TLDR
The results show that genetic remains on spider webs allow the detection of even the smallest target organisms, and demonstrate that eDNA from spider webs is useful in research of community compositions across the different domains of life, with potentially highly detailed temporal and spatial information.

Testing multiple substrates for terrestrial biodiversity monitoring using environmental DNA metabarcoding

TLDR
Test multiple sample substrates (soil, scat, plant material and bulk arthropods) to determine what organisms can be detected from each and where they overlap demonstrate the importance of selecting appropriate metabarcoding substrates when undertaking terrestrial surveys.

Spider webs as eDNA tool for biodiversity assessment of life’s domains

TLDR
The results show that genetic remains on spider webs allow the detection of even the smallest target organisms and demonstrate that eDNA from spider webs is useful in research of community compositions in different domains of life, with potentially highly detailed temporal and spatial information.

Environmental DNA metabarcoding of wild flowers reveals diverse communities of terrestrial arthropods

TLDR
The results demonstrate that it is possible to obtain information on diverse communities of insects and other terrestrial arthropods from eDNA metabarcoding of wild flowers, and represents a vast potential for addressing fundamental research questions in ecology.

DNA metabarcoding reveals broadly overlapping diets in three sympatric North American hummingbirds

TLDR
It is found that all three species of hummingbirds eat the same collection of invertebrates and flower nectars; none of them specialize on one or a few kinds of food.

Detection of herbivory: eDNA detection from feeding marks on leaves

Many techniques have been developed to investigate the interactions between plants and herbivorous insects in natural environments and are generally used to determine either (a) which plant species

One‐locus‐several‐primers: A strategy to improve the taxonomic and haplotypic coverage in diet metabarcoding studies

TLDR
The results underline the lack of predictability of both the coverage and complementarity of individual primer sets and illustrate that the OLSP strategy is especially relevant from this perspective since distinct variants within the same MOTUs were not equally detected across all primer sets.

Towards more compassionate wildlife research through the 3Rs principles: moving from invasive to non-invasive methods

Research in ecology and wildlife biology remains crucial for increasing our knowledge and improving species management and conservation in the midst of the current biodiversity crisis. However,

DNA BARCODING OF COMMON MALAYSIAN SPIDERS USING THE CYTOCHROME OXIDASE I (COI) GENE

TLDR
This study aids in an accurate identification of the selected local spider species at molecular level using the COI gene in Malaysia through DNA barcoding.

References

SHOWING 1-10 OF 16 REFERENCES

Spider Web DNA: A New Spin on Noninvasive Genetics of Predator and Prey

TLDR
A novel and promising source of noninvasive spider DNA and insect eDNA from spider webs is described, using black widow spiders fed with house crickets to extract and amplified mitochondrial DNA sequences of both spider and prey from spider web.

Sparing spiders: faeces as a non-invasive source of DNA

TLDR
The ability to amplify DNA from spider faeces with specific and general primers suggests that this sample type can be used for diagnostic PCR and sequence-based species and prey identification such as DNA barcoding and next generation sequencing, respectively.

DNA sequence from butterfly frass and exuviae

TLDR
Two species of butterfly were investigated in this study: Vanessa cardui L., the “Painted Lady”, and Pieris rapae L, the ‘Cabbage Butterfly’, and their shed skins (exuviae) may be viewed as another reservoir of DNA.

Carrion fly-derived DNA as a tool for comprehensive and cost-effective assessment of mammalian biodiversity.

TLDR
It is concluded that the analysis of fly-derived DNA can be implemented in a very rapid and cost-effective manner and will give a relatively unbiased picture of local mammal diversity.

DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.

We describe "universal" DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla:

The ecology of environmental DNA and implications for conservation genetics

TLDR
This work outlines a framework for understanding the ecology of eDNA, including the origin, state, transport, and fate of extraorganismal genetic material, and identifies frontiers of conservation-focused eDNA application where it sees the most potential for growth.

Microsatellite genotyping reveals end-Pleistocene decline in mammoth autosomal genetic variation.

TLDR
Simulation-based analysis indicates that this dramatic change in genetic composition of woolly mammoths in north-eastern Siberia from before 60 000 years ago up until the species' final disappearance c.4000 years ago suggests a rapid final extinction event.

Effect of non‐lethal sampling on life‐history traits of the protected moth Graellsia isabelae (Lepidoptera: Saturniidae)

Abstract 1. Non‐lethal genetic surveys in insects usually extract DNA from a leg or a piece of wing. Although preferable to lethal sampling, little is known about the effect of leg/wing non‐lethal

The biology of Pholcus phalangioides (Araneae, Pholcidae): predatory versatility, araneophagy and aggressive mimicry.

TLDR
Experimental evidence indicates that vision is of little or no importance in the predatory behaviour of P. phalangioides, and the behaviour of Portia, an araneophagic web-invading salticid, is compared and the results are discussed in relation to hypotheses concerning Salticid evolution.