DNA extraction from dried Schistosoma haematobium eggs isolated on nylon filters.

Abstract

Genetic studies on Schistosoma haematobium are often carried out on DNA extracted from miracidia, cercariae or adult worms. This paper presents a method for extracting DNA from S. haematobium eggs collected from urine samples and stored on nylon filters at room temperature. DNA was extracted from dried S. haematobium eggs using the DNeasy Blood & Tissue Kit (QIAGEN Sample & Assay Technologies, Copenhagen, Denmark). Selected genes were amplified using PCR to verify that DNA extraction had been successful. DNA was extracted from 45 samples and 31 had a positive PCR reaction for either or both of the two selected genes.

DOI: 10.1016/j.trstmh.2011.12.002

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@article{Reinstrup2012DNAEF, title={DNA extraction from dried Schistosoma haematobium eggs isolated on nylon filters.}, author={L Reinstrup and Anders V J\orgensen and B J Vennervald and Thomas K. Kristensen}, journal={Transactions of the Royal Society of Tropical Medicine and Hygiene}, year={2012}, volume={106 4}, pages={270-2} }