DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi

@article{Cubero2005DNAEA,
  title={DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi},
  author={Oscar F. Cubero and Ana Crespo and Jamshid Fatehi and Paul D. Bridge},
  journal={Plant Systematics and Evolution},
  year={2005},
  volume={216},
  pages={243-249}
}
This paper presents a DNA extraction method suitable for fresh, herbarium-stored, lichenized and other fungal specimens. The method is fast and reliable, comparatively inexpensive and is suitable for obtaining PCR amplification quality DNA from large numbers of samples in a short time. The method has been tested with over 300 samples ofAscochyta, Phyllosticta, Ramalina, Parmelia andPhysconia. Amplifiable fungal DNA was extracted from pure cultures, leaf lesions, whole thalli and dissected “only… CONTINUE READING

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