DNA excision-repair defect of xeroderma pigmentosum prevents removal of a class of oxygen free radical-induced base lesions ( ionizing radiation / endogenous DNA damage / purine dimers / neurological degeneration )


Plasmid DNA was rirradiated or treated with H202 in the presence of Cu2+ to generate oxygen free radical-induced lesions. Open circular DNA molecules were removed by ethidium bromide/CsCI density gradient centrifugation. The closed circular DNA fraction was treated with the Escherichia coli reagent enzymes endonuclease IH (Nth protein) and Fpg protein. This… (More)

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