DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A

@inproceedings{Huang2017DNAEE,
  title={DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A},
  author={Yung-Hsin Huang and Jianzhong Su and Yong Lei and Lorenzo Brunetti and Michael Gundry and Xiaotian Zhang and Mira Jeong and Wei Li and Margaret A. Goodell},
  booktitle={Genome Biology},
  year={2017}
}
DNA methylation has widespread effects on gene expression during development. However, our ability to assign specific function to regions of DNA methylation is limited by the poor correlation between global patterns of DNA methylation and gene expression. Here, we utilize nuclease-deactivated Cas9 protein fused to repetitive peptide epitopes (SunTag) recruiting multiple copies of antibody-fused de novo DNA methyltransferase 3A (DNMT3A) (dCas9-SunTag-DNMT3A) to amplify the local DNMT3A… CONTINUE READING
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