DNA enzyme targeting TNF-alpha mRNA improves hemodynamic performance in rats with postinfarction heart failure.

@article{Iversen2001DNAET,
  title={DNA enzyme targeting TNF-alpha mRNA improves hemodynamic performance in rats with postinfarction heart failure.},
  author={Per Ole Iversen and Gunnar Nicolaysen and Mouldy Sioud},
  journal={American journal of physiology. Heart and circulatory physiology},
  year={2001},
  volume={281 5},
  pages={H2211-7}
}
Tumor necrosis factor-alpha (TNF-alpha) probably affects the pathogenesis of heart failure. Here we have investigated the therapeutic potential of a nuclease-resistant DNA enzyme that specifically cleaves TNF-alpha mRNA. A phosphorothioate-modified DNA enzyme was designed to retain similar cleavage activity as its unmodified version, and that inhibited the expression of TNF-alpha in vitro. To test its efficacy in vivo, postinfarction congestive heart failure was induced in anesthetized rats by… CONTINUE READING