DNA-enzyme conjugate with a weak inhibitor that can specifically detect thrombin in a homogeneous medium.

@article{Shimada2011DNAenzymeCW,
  title={DNA-enzyme conjugate with a weak inhibitor that can specifically detect thrombin in a homogeneous medium.},
  author={Josui Shimada and Tatsuo Maruyama and Momoko Kitaoka and Noriho Kamiya and Masahiro Goto},
  journal={Analytical biochemistry},
  year={2011},
  volume={414 1},
  pages={
          103-8
        }
}
We present the DNA-assisted control of enzymatic activity for the detection of a target protein using a new type of DNA-enzyme conjugate. The conjugate is composed of an enzyme inhibitor to regulate enzyme activity and a DNA aptamer to be responsive toward the analyte protein. Glutathione S-transferase (GST) and thrombin were selected as a model enzyme and an analyte protein. A hexahistidine tag was genetically attached to the C terminus of the GST, and the 5' end of an oligonucleotide was… CONTINUE READING
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