DNA-directed control of enzyme-inhibitor complex formation: a modular approach to reversibly switch enzyme activity.

@article{Janssen2015DNAdirectedCO,
  title={DNA-directed control of enzyme-inhibitor complex formation: a modular approach to reversibly switch enzyme activity.},
  author={Brian M. G. Janssen and Wouter Engelen and Maarten Merkx},
  journal={ACS synthetic biology},
  year={2015},
  volume={4 5},
  pages={547-53}
}
DNA-templated reversible assembly of an enzyme-inhibitor complex is presented as a new and highly modular approach to control enzyme activity. TEM1-β-lactamase and its inhibitor protein BLIP were conjugated to different oligonucleotides, resulting in enzyme inhibition in the presence of template strand. Formation of a rigid dsDNA linker upon addition of a… CONTINUE READING