DNA demethylation facilitates the specific transcription of the mouse X-linked Tsga8 gene in round spermatids†.

  title={DNA demethylation facilitates the specific transcription of the mouse X-linked Tsga8 gene in round spermatids†.},
  author={Yongjie Lu and Shunyao Liao and Wenling Tu and Bo Yang and Shasha Liu and Xue Pei and Da-chang Tao and Yi-lu Lu and Yongxin Ma and Yuan Yang and Yunqiang Liu},
  journal={Biology of reproduction},
  volume={100 4},
Some X-linked genes necessary for spermiogenesis are specifically activated in the postmeiotic germ cells. However, the regulatory mechanism about this activation is not clearly understood. Here, we examined the potential mechanism controlling the transcriptional activation of the mouse testis specific gene A8 (Tsga8) gene in round spermatids. We observed that the Tsga8 expression was negatively correlated with the methylation level of the CpG sites in its core promoter. During spermatogenesis… 
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Tsga8 is required for spermatid morphogenesis and male fertility in mice.
It is shown that one of the genes targeted by KMT2B, Tsga8, plays an essential role in spermatid morphogenesis and is a K MT2B target that is vitally necessary for spermiogenesis and fertility.


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The data support the notion that hypomethylation and transcription factor recruitment are necessary events that precede gene activation at the early stages of spermatogenesis.
Expression and epigenomic landscape of the sex chromosomes in mouse post-meiotic male germ cells
The mouse sex chromosomes are no longer silenced but are nevertheless regulated differently than autosomes and accumulate different chromatin marks, suggesting that post-meiotic selective constraints are at the basis of the enrichment of spermatid-specific genes and of the peculiar chromatin composition of the sex chromosomes and of parts of chromosome 14.
Regulation of ALF Gene Expression in Somatic and Male Germ Line Tissues Involves Partial and Site-specific Patterns of Methylation*
Overall the results show that silencing in somatic cells is methylation-dependent and reversible and that a unique CpG-specific methylation pattern at the ALF promoter precedes expression in pachytene spermatocytes.
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The data suggest that genes activated in premeiotic stages of gametogenesis are sex-indifferent and are regulated by DNA methylation, while gene activation patterns involved in spermatocyte-specific differentiation appear to be initiated not before entry into meiosis and underlie a more complex regulation.