DNA damage by nitrogen mustard in a gene containing multiple Sp1-binding sites.

@article{Chen1999DNADB,
  title={DNA damage by nitrogen mustard in a gene containing multiple Sp1-binding sites.},
  author={Xiang Mei Chen and Carleen Cullinane and Peter J. Gray and Don R. Phillips},
  journal={Mutation research},
  year={1999},
  volume={445 1},
  pages={45-54}
}
The human cytochrome c(1) gene TATA-less promoter contains 10 Sp1-binding elements that regulate the activation of transcription of this gene. Quantitative PCR was used to show that nitrogen mustard induces DNA lesions within this Sp1-binding region following exposure of HeLa cells to clinical levels of the drug. Alkylation of the cytochrome c(1) gene in HeLa cells increased with reaction time up to 4 h following exposure to nitrogen mustard, with 50% of the lesions (approximately 0.8/kb… CONTINUE READING

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