DNA damage and repair measured in different genomic regions using the comet assay with fluorescent in situ hybridization.

@article{Horvthov2004DNADA,
  title={DNA damage and repair measured in different genomic regions using the comet assay with fluorescent in situ hybridization.},
  author={Eva Horv{\'a}thov{\'a} and M{\'a}ria Du{\vs}insk{\'a} and Sergey Shaposhnikov and Andrew R Collins},
  journal={Mutagenesis},
  year={2004},
  volume={19 4},
  pages={269-76}
}
The comet assay is a sensitive method for measuring DNA strand breaks in eukaryotic cells. After embedding in agarose, cells are lysed and electrophoresed at high pH. DNA loops containing breaks (in which supercoiling is relaxed) escape from the nucleoid comet head to form a tail. Oligonucleotide probes were designed for 5' and 3' regions of the genes for dihydrofolate reductase (DHFR) and O6-methylguanine DNA methyltransferase (MGMT), both from the Chinese hamster, and the human tumour… CONTINUE READING