DNA damage and anti-tumor activity induced by Zn, Ag and Co containing meso- tetra(4-N-oxyethylpyridyl)porphyrins in vivo

  title={DNA damage and anti-tumor activity induced by Zn, Ag and Co containing meso- tetra(4-N-oxyethylpyridyl)porphyrins in vivo},
  author={Nelli H. Karapetyan and Lusya V. Torosyan and Gayane V. Ananyan and Rafael Muradyan},
  journal={Journal of Porphyrins and Phthalocyanines},
In the present study, the activity of the new water-soluble cationic meso-tetra(4-N-oxyethylpyridyl)porphyrin and its Zn, Ag and Co metal derivatives as anti-tumor agents was explored. The tumor was induced by 7,12-dimethylbenz[a]antracene (DMBA) on rats of Wistar strain. The levels of DNA damage induced by porphyrins T4OEPyP, AgT4OEPyP, ZnT4OEPyP and CoT4OEPyP in tumor tissue were analyzed. Thermodynamic parameters of DNA were investigated by thermal melting method and differential scanning… 
pH-dependent complex formation of Zn-meso-tetra(4-N-hydroxyethylpyridyl) porphyrin with cancer DNA
The results show that the binding constant and the exclusion parameter of ZnTOEPyP4 with DNA strongly depend upon the pH, which is higher than with normal DNA and the complex formation between Zn-meso-tetra(4-N-hydroxyethylpyridyl) porphyrin and cancer DNA is higher.
Influence of Amino Acids Shiff Bases on Irradiated DNA Stability In Vivo
It was shown that pre-treatment of rats with Mn(II) complexes approximates the melting parameters to norm and improves the DNA characteristics, and separate DNA fragments were revealed in DNA samples isolated from irradiated animals.
DNA‐Targeted Inhibition of MGMT
The cationic porphyrin 5,10,15,20‐tetrakis (diisopropyl‐guanidine)‐21H,23H‐porphine (DIGPor) selectively binds to DNA containing O6‐methylguanine (O6‐MeG) and inhibits the DNA repair enzyme


In Vitro Testing of Cyto- and Genotoxicity of New Porphyrin Water-Soluble Metal Derivatives
The metalloporphyrins tested meet at least two important demands to potential anticancer drugs as they combine the cytotoxicity with low genotoxicity and did not cause chromosome fragmentation in K-562 cell line at as high concentrations as IC50.
Interactions of Meso-tetra-(4-N-oxyethylpyridyl) Porphyrin, Its 3-N Analog and Their Metallocomplexes with Duplex DNA
Results indicate that (4N-oxyethylpyridyl) porphyrins intercalate more preferably in the structure of duplex DNA and have weaker external binding than 3N-porphyrins.
Complexes of bovine serum albumin with water-soluble Cu and Co containing cationic meso-tetra(4-N-oxyethylpyridyl)-porphyrins
Bovine serum albumin complexes with water-soluble cationic porphyrins, Cu- and Co-meso-tetra(4-N-hydroxyethylpyridyl)porphyrins (CuT4OEPyP, CoT4OEPyP), and their 3-N-analogs,
Photodynamic effects of a cationic mesosubstituted porphyrin in cell cultures.
The photobiological activity of mesotetra (4N-methylpyridyl) porphine (T4MPyP) has been studied in a mammalian cell line in vitro and cellular DNA-photodamage was studied under sublethal conditions by the use of the micronucleus test.
Efficacy of potential chemopreventive agents on rat colon aberrant crypt formation and progression.
Certain phytochemicals, antihistamines, non-steroidal anti-inflammatory drugs and retinoids show unique preclinical promise for chemoprevention of colon cancer, with the latter two drug classes particularly effective in the post-initiation phase of carcinogenesis.
Photodynamic therapy in oncology: mechanisms and clinical use.
  • H. Pass
  • Medicine, Biology
    Journal of the National Cancer Institute
  • 1993
Clinically, this therapy has been chiefly used for palliation of endobronchial and esophageal obstruction, as well as for treatment of bladder carcinomas, skin malignancies, and brain tumors, and the future of PDT rests in defining its use either as an intraoperative adjuvant to marginal surgical procedures or as a primary treatment for superficial malignancy.
Apoptosis: biochemical aspects and clinical implications.
  • F. Kiechle, Xinbo Zhang
  • Biology, Chemistry
    Clinica chimica acta; international journal of clinical chemistry
  • 2002
Photophysical and photobiological processes in the photodynamic therapy of tumours.
  • M. Ochsner
  • Biology, Medicine
    Journal of photochemistry and photobiology. B, Biology
  • 1997
High-resolution mapping of DNA hypermethylation and hypomethylation in lung cancer
It is found that extensive DNA hypomethylation in tumors occurs specifically at repetitive sequences, including short and long interspersed nuclear elements and LTR elements, segmental duplications, and subtelomeric regions, but single-copy sequences rarely become demethylated.