DNA constructs designed to produce short hairpin, interfering RNAs in transgenic mice sometimes show early lethality and an interferon response.

@article{Cao2005DNACD,
  title={DNA constructs designed to produce short hairpin, interfering RNAs in transgenic mice sometimes show early lethality and an interferon response.},
  author={Wen Cao and Robert J Hunter and Diana W Strnatka and Charlene A. Mcqueen and Robert P. Erickson},
  journal={Journal of applied genetics},
  year={2005},
  volume={46 2},
  pages={217-25}
}
Arylamine N-acetyltransferase (NAT) genes were targeted for inhibition using short hairpin RNA (shRNA) using two different RNA polymerase III promoters. Constructs were developed for NAT1 and NAT2, the endogenous mouse genes, and for human NAT1. There were fetal and neonatal deaths with these constructs, perhaps due in part to an interferon response as reflected in increases in oligoadenylate synthetase I mRNA levels. Seven out of 8 founders with the U6 promoter generated offspring but only 2… CONTINUE READING
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Activation of the interferon system by shortinterfering RNAs

  • CA Sledz, M Holko, MJ deVeer, RH Silverman, Williams BRG
  • Nature Cell Biol
  • 2003
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