DNA condensation monitoring after interaction with hoechst 33258 by atomic force microscopy and fluorescence spectroscopy.

@article{Saito2004DNACM,
  title={DNA condensation monitoring after interaction with hoechst 33258 by atomic force microscopy and fluorescence spectroscopy.},
  author={Masato Saito and Masaaki Kobayashi and Shin-ichiro Iwabuchi and Yasutaka Morita and Yuzuru Takamura and Eiichi Tamiya},
  journal={Journal of biochemistry},
  year={2004},
  volume={136 6},
  pages={813-23}
}
DNA condensation was only observed after the addition of Hoechst 33258 (H33258) among various types of DNA binding molecules. The morphological structural change of DNA was found to depend on the H33258 concentration. On comparison of fluorescence spectrum measurements with AFM observation, it was found that fluorescence quenching of DNA-H33258 complexes occurred after DNA condensation. Additionally, we showed that DNA condensation by H33258 was independent of sequence selectivity or binding… CONTINUE READING

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