DNA cloning in Bacillus subtilis. III. Efficiency of random-segment cloning and insertional inactivation vectors.

@article{Michel1980DNACI,
  title={DNA cloning in Bacillus subtilis. III. Efficiency of random-segment cloning and insertional inactivation vectors.},
  author={B{\'e}n{\'e}dicte Michel and Emanuela Palla and Brigitte Niaudet and S. Dusko Ehrlich},
  journal={Gene},
  year={1980},
  volume={12 1-2},
  pages={
          147-54
        }
}
Random segments of Bacillus amyloliquefaciens and yeast Saccharomyces cerevisiae DNA were used to determine two parameters pertinent to cloning in Bacillus subtilis, the yield of hybrids and the mean size of cloned segments. 10(3) to 10(4) hybrids/micrograms of DNA segments were obtained. Hybrids represented 11--18% of transformants. Mean m. wt. of cloned DNA segments was about 1 x 10(6), substantially lower than 3 x 10(6) found for donor DNAs after digestion with restriction endonucleases. We… CONTINUE READING

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