DNA breaks in hypermutating immunoglobulin genes: evidence for a break-and-repair pathway of somatic hypermutation.

@article{Kong2001DNABI,
  title={DNA breaks in hypermutating immunoglobulin genes: evidence for a break-and-repair pathway of somatic hypermutation.},
  author={Q Kong and Nancy Maizels},
  journal={Genetics},
  year={2001},
  volume={158 1},
  pages={369-78}
}
To test the hypothesis that immunoglobulin gene hypermutation in vivo employs a pathway in which DNA breaks are introduced and subsequently repaired to produce mutations, we have used a PCR-based assay to detect and identify single-strand DNA breaks in lambda1 genes of actively hypermutating primary murine germinal center B cells. We find that there is a two- to threefold excess of breaks in lambda1 genes of hypermutating B cells, relative to nonhypermutating B cells, and that 1.3% of germinal… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 23 references

Breaksite batch mapping , a rapid method for assay and identification of DNA breaksites in mamma - Brenner , S . , and C . Milstein , 1966 Origin of antibody variation

N. Maizels
Nature • 2001
View 3 Excerpts
Highly Influenced

PMS 2 - deficiency diminishes hyper - activating gene ( RAG ) 1 activity

N. Maizels
J . Exp . Med . • 1999
View 3 Excerpts
Highly Influenced

Discriminating mutation of a l 1 transgene in young but not older mice

A. G. Betz, M. S. Neuberger, C. Milstein
Mol . Immunol . • 1993
View 3 Excerpts
Highly Influenced

DNA double - strand breaks in immunoglobulin genes based mechanisms for somatic hypermutation

R. S. Harris, N. Maizels, +3 authors K. Rajewsky
Immunol . Rev . • 2000

The nucleotide - replacement

T. B. Kepler
Curr . Top . Microbiol . Immunol . • 2000
View 2 Excerpts

Characteristics et al . , 1998 b Somatic hypermutation introduces insertions andof sequences around individual nucleotide substitutions in IgVH deletions into immunoglobulin V genes

O. de Bouteiller, K. Potter, J. BanchereauSpencer, M. Dunn, D. K. Dunn-Walters
J . Exp . Med . Immunol . Rev . • 1999

Muta - tional pattern of the nurse shark antigen receptor gene ( NAR ) et al . , 1998 Monitoring and interpreting the intrinsic features of somatic hypermutation

M. R. Ehrenstein, N. Klix, +3 authors J. Cerny
Immunol . Rev . • 1999

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