DNA breakage detection-FISH (DBD-FISH) in human spermatozoa: technical variants evidence different structural features.

@article{Fernndez2000DNABD,
  title={DNA breakage detection-FISH (DBD-FISH) in human spermatozoa: technical variants evidence different structural features.},
  author={Jos{\'e} L. Fern{\'a}ndez and Fernando V{\'a}zquez-Gund{\'i}n and Alexie Delgado and Vicente Goyanes and Juan Ramiro-D{\'i}az and Jes{\'u}s Francisco Preciado de la Torre and Jaime Gos{\'a}lvez},
  journal={Mutation research},
  year={2000},
  volume={453 1},
  pages={77-82}
}
Non-irradiated and X-irradiated (80 Gy) human spermatozoa were processed for in situ DNA breakage detection-FISH (DBD-FISH) of the whole genome, following two alternative variations of the basic technique. In the first, cells were initially incubated in the alkaline unwinding solution for transformation of DNA breaks into single-stranded DNA (ssDNA) to be hybridized, followed by the lysing solutions for protein removal. In the second, incubation in the lysing solutions was carried out before… CONTINUE READING