DNA binding fluorochromes as probes for histone H1-chromatin interactions in situ.


We have investigated using the DNA binding fluorochromes 7-aminoactinomycin (7-AAMD) and 4',6-diamidino-2-phenylindole (DAPI) as cytochemical probes for linker histone (H1)-chromatin interactions in situ. Human lymphocytes, permeabilized with digitonin, were exposed to increasing concentrations of sodium chloride to remove ionically bound H1 from the nuclei… (More)