DNA binding activity of the baculovirus late expression factor PP31.

Abstract

PP31 is a baculovirus protein that is essential for viral late gene expression. To study the role of PP31 in late transcription in vitro, it was purified from infected insect cells. A combination of heparin affinity, cation exchange chromatography, and gel filtration was used to purify native non-tagged protein. Nearly 5 mg of PP31 was obtained from 95 mg… (More)

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Cite this paper

@article{Guarino2002DNABA, title={DNA binding activity of the baculovirus late expression factor PP31.}, author={Linda A. Guarino and Toni-Ann Mistretta and Wen Dong}, journal={Virus research}, year={2002}, volume={90 1-2}, pages={187-95} }