DNA-based detection of the fungal pathogen Geomyces destructans in soils from bat hibernacula

@article{Lindner2011DNAbasedDO,
  title={DNA-based detection of the fungal pathogen Geomyces destructans in soils from bat hibernacula},
  author={Daniel L Lindner and Andrea Gargas and Jeffrey M. Lorch and Mark T. Banik and Jessie A. Glaeser and Thomas H. Kunz and David S. Blehert},
  journal={Mycologia},
  year={2011},
  volume={103},
  pages={241 - 246}
}
White-nose syndrome (WNS) is an emerging disease causing unprecedented morbidity and mortality among bats in eastern North America. The disease is characterized by cutaneous infection of hibernating bats by the psychrophilic fungus Geomyces destructans. Detection of G. destructans in environments occupied by bats will be critical for WNS surveillance, management and characterization of the fungal lifecycle. We initiated an rRNA gene region-based molecular survey to characterize the distribution… Expand
A culture-based survey of fungi in soil from bat hibernacula in the eastern United States and its implications for detection of Geomyces destructans, the causal agent of bat white-nose syndrome
TLDR
Investigation of the diversity of fungi in soil samples collected from 24 bat hibernacula in the eastern United States found Geomyces species were one of the most abundant and diverse groups cultured, representing approximately 33% of all isolates. Expand
Distribution and Environmental Persistence of the Causative Agent of White-Nose Syndrome, Geomyces destructans, in Bat Hibernacula of the Eastern United States
TLDR
Analysis of sediment samples collected from 55 bat hibernacula both within and outside the known range of WNS indicates that the distribution of G. destructans is correlated with disease in hibernating bats and supports the hypothesis that the fungus is likely an exotic species in North America. Expand
Short title: Fungi in bat hibernacula A culture-based survey of fungi in soil from bat hibernacula in the eastern United States and its implications for detection of Geomyces destructans, the causal agent of bat white-nose syndrome
TLDR
Investigation of the diversity of fungi in soil samples collected from 24 bat hibernacula in the eastern United States found Geomyces species were one of the most abundant and diverse groups cultured, representing approximately 33% of all isolates. Expand
Highly Sensitive Quantitative PCR for the Detection and Differentiation of Pseudogymnoascus destructans and Other Pseudogymnoascus Species
TLDR
This work presents a dual-probe real-time quantitative PCR assay capable of detecting and differentiating P. destructans from closely related fungi in environmental samples from North America, and is capable of rapid low-level detection from various sampling media. Expand
Surveillance of Geomyces destructans in Swedish bats and Bat Hibernacula
TLDR
The widespread distribution of G. destructans in Europe and the absence of bat mortalities in Europe suggests that even if G.destructans was to be found in Sweden it would presently not be considered to be an immediate threat to Swedish bat populations. Expand
Psychrophilic and Psychrotolerant Fungi on Bats and the Presence of Geomyces spp. on Bat Wings Prior to the Arrival of White Nose Syndrome
TLDR
It is confirmed that a large diversity of fungi, including several Geomyces isolates, occurs on bats prior to the arrival of WNS, and all Geomyce strains isolated are psychrotolerant, unlike G. destructans, which is a true psychrophile. Expand
Bat white-nose syndrome: a real-time TaqMan polymerase chain reaction test targeting the intergenic spacer region of Geomyces destructans
TLDR
A real-time TaqMan PCR test that detects DNA from G. destructans by targeting a portion of the multicopy intergenic spacer region of the rRNA gene complex, which is highly sensitive and provides a valuable tool for WNS diagnostics and research. Expand
Phylogenetic evaluation of Geomyces and allies reveals no close relatives of Pseudogymnoascus destructans, comb. nov., in bat hibernacula of eastern North America.
TLDR
DNA sequence data for the internal transcribed spacer region, nuclear large subunit (LSU) rDNA, MCM7, RPB2, and TEF1 from a diverse array of Geomyces and allies that included isolates recovered from bat hibernacula as well as those that represent important type species provide further support to the hypothesis that this pathogen is non-native and invasive in eastern North America. Expand
Clonal Expansion of the Pseudogymnoascus destructans Genotype in North America Is Accompanied by Significant Variation in Phenotypic Expression
TLDR
Pseudogymnoascus destructans is the causative agent of an emerging infectious disease that threatens populations of several North American bat species and is characterized by a cutaneous infection with P. destructans causing erosions and ulcers in the skin of nose, ears and/or wings of bats. Expand
Skin Lesions in European Hibernating Bats Associated with Geomyces destructans, the Etiologic Agent of White-Nose Syndrome
White-nose syndrome (WNS) has claimed the lives of millions of hibernating insectivorous bats in North America. Its etiologic agent, the psychrophilic fungus Geomyces destructans, causes skin lesionsExpand
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