DNA barcoding of earthworms ( Eisenia fetida / andrei complex ) from 28 ecotoxicological test laboratories

  title={DNA barcoding of earthworms ( Eisenia fetida / andrei complex ) from 28 ecotoxicological test laboratories},
  author={J{\"o}rg R{\"o}mbke and Manuel Aira and Thierry Backeljau and Karin Breugelmans and Jorge I. Dom{\'i}nguez and Elisabeth Funke and Nadin Graf and Mehrdad Hajibabaei and Marcos P{\'e}rez-Losada and Pablo G. Porto and R{\"u}diger M. Schmelz and Joaqu{\'i}n Vierna and Ant{\'o}n Vizca{\'i}no and Markus Pfenninger},
Plenty of evidence indicates that the earthworms Eisenia fetida (Savigny, 1826) and Eisenia andrei Bouché, 1972 (Lumbricidae) can be distinguished by morphological, physiological and molecular traits. However, the morphological differences alone do not allow to correctly identify these taxa. This may be a serious problem for the reliability of the standard ecotoxicological tests for which these worms are used. Recently, DNA barcoding (i.e., sequencing of a standard mtDNA COI gene fragment for… CONTINUE READING
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